The Chemical Biology Platform can employ a variety of techniques in various microtiter plate-based formats (96/384 wells). Assays can be conducted with either purified protein, membranes, living cells (mammalian, bacteria, yeast) using a variety of readouts, including:
- Radioactivity-based assays
- Radioligand binding
- Enzymatic and/or signal transduction assays
- Fluorescence intensity
- FRET
- Fluorescence polarisation/anisotropy
- Time-resolved fluorescence
- Tr-FRET
- Luminescence
- Flash
- Glow
- BRET
- Protein fragment complementation
- Absorbance
- Surface Plasmon Resonance
- Isothermal titration calorimetry
- Virtual screening technologies
- Chemoinformatics analyses
- Protein homology modeling
- Pharmacophore modeling
- Molecular docking simulations
- Ligand binding site comparison
For most screening requests, these different technologies will offer suitable options to conduct a reliable screen.