New set of fluorescent proteins (FPs) outperform conventional FPs in yeast

Researchers of the VU and UvA published in Scientific Reports the results of 27 systematically tested FPs.

02/26/2019 | 11:02 AM

Fluorescent proteins (FPs) are widely used to study proteins and processes in single cells, in many different organisms, but little is known about their performance in other species than bacteria or mammalian cells. Information about FP behaviour in other species is essential to ensure the best readout without any artefacts. In a recent study published in Scientific Reports, Botman (AIMMS) and colleagues therefore systematically tested 27 FPs in Baker’s yeast, a very important model organism for biotechnology and cell biology. It turned out that many FPs showed different performance in yeast than in mammalian cells. The authors optimised FPs for the use in yeast and show that the new available set of FPs outperform the conventional FPs, which improves experimental signal readout, opening new experimental possibilities. This work is a fruitful collaboration between groups of the VU (Teusink) and the UvA (Goedhart).

Find the open access article here.

Botman Yeast FP

Figure 1. Example of yeast cells expressing the bright and codon-optimized ymTurquoise2 (left) or ymNeongreen (right) fluorescent proteins.